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b cells  (Miltenyi Biotec)


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    Miltenyi Biotec b cells
    B Cells, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 700 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/b+cell+isolation+kit+ii/B+Cell+Isolation+Kit+II%2C+human/pmc13421502-311-15-26
    Average 96 stars, based on 700 article reviews
    b cells - by Bioz Stars, 2026-09
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    Related Articles

    Isolation:

    Article Title: ATOR-4066, a Bispecific Antibody Targeting CD40 and CEACAM5, Induces Strong Myeloid and T Cell–Dependent Tumor Immunity and Synergizes with PD-1 Blockade
    Article Snippet: .. For the B-cell assay, B cells were enriched from PBMCs isolated from leukocyte concentrates obtained from healthy donors using the B Cell Isolation Kit II, human (130-091-151, Miltenyi Biotec). .. B cells (50,000 cells/well) were incubated with IL4 (10 ng/mL, 200-04, Gibco) in 96-well plates together with CHO-CEACAM5 cells (60,000 cells/well), CHO-WT cells (60,000 cells/well), soluble CEACAM5 (1 or 5 μg/mL), or the combination of CHO-CEACAM5 and soluble CEACAM5, with or without ATOR-4066 (1 nmol/L).

    Article Title: CD8 + T cell depletion promotes human Tph/Tfh cell proliferation and Sjögren syndrome–like symptoms in PBMC-based humanized mice
    Article Snippet: .. B cells were isolated using the B-Cell Isolation Kit II, human (Miltenyi Biotec), and CD4 + T cells were isolated using the CD4 + T Cell Isolation Kit, human (Miltenyi Biotec), following the manufacturer’s protocols. .. CD8 + T cells derived from hPBMC-transferred NSG mice or control CD8 + T cells (5 × 10 4 ) were cultured with either B (1 × 10 4 ) or CD4 + T cells (1 × 10 4 ) in 96-well, round-bottom plates precoated with anti-CD3 antibody (5 μg/mL; clone OKT3, BioLegend).

    Article Title: Interferon-mediated NK cell activation increases cytolytic activity against T follicular helper cells and limits antibody response to SARS-CoV-2.
    Article Snippet: .. B cells were isolated from cryopreserved PBMCs using a Miltenyi B cell Isolation Kit II, human (Miltenyi; 130-091-151), according to the manufacturer’s recommendation. ..

    Article Title: CD8+ T cell depletion promotes human Tph/Tfh cell proliferation and Sjögren syndrome-like symptoms in PBMC-based humanized mice.
    Article Snippet: .. B cells were isolated using the B-Cell Isolation Kit II, human (Miltenyi Biotec), and CD4+ T cells were isolated using the CD4+ T Cell Isolation Kit, human (Miltenyi Biotec), following the manufacturer’s protocols. .. CD8+ T cells derived from hPBMC-transferred NSG mice or control CD8+ T cells (5 × 104) were cultured with either B (1 × 104) or CD4+ T cells (1 × 104) in 96-well, round-bottom plates precoated with anti-CD3 antibody (5 μg/mL; clone OKT3, BioLegend).

    Article Title: Inhibition of KDM5A/B promotes antitumor innate immune responses in HHV-8/KSHV-positive B-cell lymphomas
    Article Snippet: Human primary cells, including peripheral blood mononuclear cells (PBMCs) (STEMCELL Technologies) as well as the isolated B cells, were maintained in RPMI 1640 complete medium (RPMI 1640 basic, 1× MEM Non-Essential Amino Acids Solution, 1× Sodium Pyruvate, and 20 mM HEPES) supplemented with human recombinant IL-2 (rIL-2, Roche) at 30 U/mL. .. Human CD19 + CD3 − primary B cells were isolated from PBMCs using the B cell Isolation Kit II (Miltenyi Biotec, Cat# 130-091-151) according to the manufacturer’s protocol. .. Doxycycline was purchased from Fisher Scientific (Cat# BP2653-1).

    Cell Isolation:

    Article Title: ATOR-4066, a Bispecific Antibody Targeting CD40 and CEACAM5, Induces Strong Myeloid and T Cell–Dependent Tumor Immunity and Synergizes with PD-1 Blockade
    Article Snippet: .. For the B-cell assay, B cells were enriched from PBMCs isolated from leukocyte concentrates obtained from healthy donors using the B Cell Isolation Kit II, human (130-091-151, Miltenyi Biotec). .. B cells (50,000 cells/well) were incubated with IL4 (10 ng/mL, 200-04, Gibco) in 96-well plates together with CHO-CEACAM5 cells (60,000 cells/well), CHO-WT cells (60,000 cells/well), soluble CEACAM5 (1 or 5 μg/mL), or the combination of CHO-CEACAM5 and soluble CEACAM5, with or without ATOR-4066 (1 nmol/L).

    Article Title: CD8 + T cell depletion promotes human Tph/Tfh cell proliferation and Sjögren syndrome–like symptoms in PBMC-based humanized mice
    Article Snippet: .. B cells were isolated using the B-Cell Isolation Kit II, human (Miltenyi Biotec), and CD4 + T cells were isolated using the CD4 + T Cell Isolation Kit, human (Miltenyi Biotec), following the manufacturer’s protocols. .. CD8 + T cells derived from hPBMC-transferred NSG mice or control CD8 + T cells (5 × 10 4 ) were cultured with either B (1 × 10 4 ) or CD4 + T cells (1 × 10 4 ) in 96-well, round-bottom plates precoated with anti-CD3 antibody (5 μg/mL; clone OKT3, BioLegend).

    Article Title: Interferon-mediated NK cell activation increases cytolytic activity against T follicular helper cells and limits antibody response to SARS-CoV-2.
    Article Snippet: .. B cells were isolated from cryopreserved PBMCs using a Miltenyi B cell Isolation Kit II, human (Miltenyi; 130-091-151), according to the manufacturer’s recommendation. ..

    Article Title: CD8+ T cell depletion promotes human Tph/Tfh cell proliferation and Sjögren syndrome-like symptoms in PBMC-based humanized mice.
    Article Snippet: .. B cells were isolated using the B-Cell Isolation Kit II, human (Miltenyi Biotec), and CD4+ T cells were isolated using the CD4+ T Cell Isolation Kit, human (Miltenyi Biotec), following the manufacturer’s protocols. .. CD8+ T cells derived from hPBMC-transferred NSG mice or control CD8+ T cells (5 × 104) were cultured with either B (1 × 104) or CD4+ T cells (1 × 104) in 96-well, round-bottom plates precoated with anti-CD3 antibody (5 μg/mL; clone OKT3, BioLegend).

    Article Title: Multi-omics reveal vitamin D regulation of immune-gut microbiome interactions and tolerogenic pathways in inflammatory bowel disease
    Article Snippet: BD RhapsodyTM WTA Amplification Kit , BD Biosciences , Catalog No. 633801. .. Miltenyi B Cell Isolation Kit II, human , Miltenyi , Catalog no. 130-091-151). .. Miltenyi CD304 BDCA-4/Neuropilin-1 MicroBead Kit, human , Miltenyi , Catalog no. 130-090-532.

    Article Title: Methotrexate Exhibits a Dual Role in Regulation of CD4 + T Follicular Helper (Tfh) Cell Differentiation and Activity.
    Article Snippet: ECAR and fatty acid oxidation of in vitro differentiated Tfh cells was measured using an extracellular acidification kit (abcam ab197244) and a fatty acid oxidation complete assay kit (abcam ab222944) according to the manufacturer's protocol, in the presence and absence of MTX treatment as indicated by the time points observed in the fluorimeter. .. Autologous B cells were enriched from PBMCs using the B Cell Isolation Kit II, human (Miltenyi Biotec: 130- 091- 186) following the manufacturer's instructions. in vitro differentiated human CD4+ T cells after 5 days (2 days activation +3 days differentiation in the presence of Tfh polarising cytokines) were co- cultured with autologous B cells (in a 1:1 ratio) for 6 days in 96- well U- bottom plates in complete RPMI medium supplemented with heat- inactivated 10% FBS in the presence of superantigen Staphylococcus enterotoxin B (100 ng/mL) (Sigma- Aldrich S4881). .. The amount of IgG produced in the culture supernatant was quantified by ELISA as per the manufacturer's instructions using the Human IgG ELISA Kit (Abcam: ab195215).

    Article Title: Inhibition of KDM5A/B promotes antitumor innate immune responses in HHV-8/KSHV-positive B-cell lymphomas
    Article Snippet: Human primary cells, including peripheral blood mononuclear cells (PBMCs) (STEMCELL Technologies) as well as the isolated B cells, were maintained in RPMI 1640 complete medium (RPMI 1640 basic, 1× MEM Non-Essential Amino Acids Solution, 1× Sodium Pyruvate, and 20 mM HEPES) supplemented with human recombinant IL-2 (rIL-2, Roche) at 30 U/mL. .. Human CD19 + CD3 − primary B cells were isolated from PBMCs using the B cell Isolation Kit II (Miltenyi Biotec, Cat# 130-091-151) according to the manufacturer’s protocol. .. Doxycycline was purchased from Fisher Scientific (Cat# BP2653-1).

    In Vitro:

    Article Title: Methotrexate Exhibits a Dual Role in Regulation of CD4 + T Follicular Helper (Tfh) Cell Differentiation and Activity.
    Article Snippet: ECAR and fatty acid oxidation of in vitro differentiated Tfh cells was measured using an extracellular acidification kit (abcam ab197244) and a fatty acid oxidation complete assay kit (abcam ab222944) according to the manufacturer's protocol, in the presence and absence of MTX treatment as indicated by the time points observed in the fluorimeter. .. Autologous B cells were enriched from PBMCs using the B Cell Isolation Kit II, human (Miltenyi Biotec: 130- 091- 186) following the manufacturer's instructions. in vitro differentiated human CD4+ T cells after 5 days (2 days activation +3 days differentiation in the presence of Tfh polarising cytokines) were co- cultured with autologous B cells (in a 1:1 ratio) for 6 days in 96- well U- bottom plates in complete RPMI medium supplemented with heat- inactivated 10% FBS in the presence of superantigen Staphylococcus enterotoxin B (100 ng/mL) (Sigma- Aldrich S4881). .. The amount of IgG produced in the culture supernatant was quantified by ELISA as per the manufacturer's instructions using the Human IgG ELISA Kit (Abcam: ab195215).

    Activation Assay:

    Article Title: Methotrexate Exhibits a Dual Role in Regulation of CD4 + T Follicular Helper (Tfh) Cell Differentiation and Activity.
    Article Snippet: ECAR and fatty acid oxidation of in vitro differentiated Tfh cells was measured using an extracellular acidification kit (abcam ab197244) and a fatty acid oxidation complete assay kit (abcam ab222944) according to the manufacturer's protocol, in the presence and absence of MTX treatment as indicated by the time points observed in the fluorimeter. .. Autologous B cells were enriched from PBMCs using the B Cell Isolation Kit II, human (Miltenyi Biotec: 130- 091- 186) following the manufacturer's instructions. in vitro differentiated human CD4+ T cells after 5 days (2 days activation +3 days differentiation in the presence of Tfh polarising cytokines) were co- cultured with autologous B cells (in a 1:1 ratio) for 6 days in 96- well U- bottom plates in complete RPMI medium supplemented with heat- inactivated 10% FBS in the presence of superantigen Staphylococcus enterotoxin B (100 ng/mL) (Sigma- Aldrich S4881). .. The amount of IgG produced in the culture supernatant was quantified by ELISA as per the manufacturer's instructions using the Human IgG ELISA Kit (Abcam: ab195215).

    Cell Culture:

    Article Title: Methotrexate Exhibits a Dual Role in Regulation of CD4 + T Follicular Helper (Tfh) Cell Differentiation and Activity.
    Article Snippet: ECAR and fatty acid oxidation of in vitro differentiated Tfh cells was measured using an extracellular acidification kit (abcam ab197244) and a fatty acid oxidation complete assay kit (abcam ab222944) according to the manufacturer's protocol, in the presence and absence of MTX treatment as indicated by the time points observed in the fluorimeter. .. Autologous B cells were enriched from PBMCs using the B Cell Isolation Kit II, human (Miltenyi Biotec: 130- 091- 186) following the manufacturer's instructions. in vitro differentiated human CD4+ T cells after 5 days (2 days activation +3 days differentiation in the presence of Tfh polarising cytokines) were co- cultured with autologous B cells (in a 1:1 ratio) for 6 days in 96- well U- bottom plates in complete RPMI medium supplemented with heat- inactivated 10% FBS in the presence of superantigen Staphylococcus enterotoxin B (100 ng/mL) (Sigma- Aldrich S4881). .. The amount of IgG produced in the culture supernatant was quantified by ELISA as per the manufacturer's instructions using the Human IgG ELISA Kit (Abcam: ab195215).



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    (A) B cells were cultured with the vehicle or mixed stimulatory molecules, including the anti-mouse IgM antibody, IL-21, IFN-γ, anti-CD40 antibody, or R848. (B) CD11c levels in unstimulated B cells. (C) Rgs13 and T-bet levels were compared with and without stimulation. (D) Rgs13 and T-bet levels in CD11c + and CD11c − stimulated B cells. (E) ABC proportions in stimulated B cells. ** p ≤ 0.01, *** p ≤ 0.001, and ns (not significant), determined via unpaired (B and E) or paired (C and D) t -test. ABC, age-associated B cell; KO, knockout; <t>MACS,</t> <t>magnetic-activated</t> cell sorting; WT, wild-type. Created in BioRender under a CC BY license, with permission from BioRender. Hiwa, R. (2026) https://BioRender.com/4xpndar .
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    Image Search Results


    (A) B cells were cultured with the vehicle or mixed stimulatory molecules, including the anti-mouse IgM antibody, IL-21, IFN-γ, anti-CD40 antibody, or R848. (B) CD11c levels in unstimulated B cells. (C) Rgs13 and T-bet levels were compared with and without stimulation. (D) Rgs13 and T-bet levels in CD11c + and CD11c − stimulated B cells. (E) ABC proportions in stimulated B cells. ** p ≤ 0.01, *** p ≤ 0.001, and ns (not significant), determined via unpaired (B and E) or paired (C and D) t -test. ABC, age-associated B cell; KO, knockout; MACS, magnetic-activated cell sorting; WT, wild-type. Created in BioRender under a CC BY license, with permission from BioRender. Hiwa, R. (2026) https://BioRender.com/4xpndar .

    Journal: PLOS One

    Article Title: Significance of RGS13 expression in lupus B cells

    doi: 10.1371/journal.pone.0348945

    Figure Lengend Snippet: (A) B cells were cultured with the vehicle or mixed stimulatory molecules, including the anti-mouse IgM antibody, IL-21, IFN-γ, anti-CD40 antibody, or R848. (B) CD11c levels in unstimulated B cells. (C) Rgs13 and T-bet levels were compared with and without stimulation. (D) Rgs13 and T-bet levels in CD11c + and CD11c − stimulated B cells. (E) ABC proportions in stimulated B cells. ** p ≤ 0.01, *** p ≤ 0.001, and ns (not significant), determined via unpaired (B and E) or paired (C and D) t -test. ABC, age-associated B cell; KO, knockout; MACS, magnetic-activated cell sorting; WT, wild-type. Created in BioRender under a CC BY license, with permission from BioRender. Hiwa, R. (2026) https://BioRender.com/4xpndar .

    Article Snippet: B cells were further separated from the peripheral blood mononuclear cells via magnetic-activated cell sorting (MACS; B Cell Isolation Kit II human; Miltenyi Biotec, Bergisch Gladbach, Germany).

    Techniques: Cell Culture, Knock-Out, FACS

    Patients with BENTA have aberrant B-cell subset distributions and show B-cell–intrinsic differentiation defects. A, IgD vs CD27 expression ( upper panel ) and CD24 vs CD38 expression ( lower panel ) were assessed within gated CD19 + B cells in PBMC samples from a healthy control (HC), I-1 (WT/R331P), P1 (R331P/R331P), and P2 (WT/G126D) by CyTOF analysis. B, Quantitation of switched memory (IgD − CD27 + ), nonswitched memory (IgD + CD27 + ), naive (CD27 − IgD + ), and transitional (CD24 hi CD38 hi ) B-cell frequencies in healthy donors, including adult healthy controls (HC, n = 12; dark blue symbols ), healthy pediatric controls (HP, n = 2; light blue symbols ), and the healthy father of P1 (WT/R331P; yellow symbol ) ( left bar ), and 2 patients with BENTA, P1 (R331P/R331P; red symbol ) and P2 (WT/G126D; orange symbol ) ( right bar ), by CyTOF analysis. * P < .05 (1-way ANOVA). C, Naive B cells were enriched from PBMC samples of 3 healthy controls (HC1, HC2, and HC3), I-1 (WT/R331P), P1 (R331P/R331P), and P2 (WT/G126D), and expression levels of IgD and CD27 were assessed by flow cytometry immediately following isolation (day 0; right panel ) and after 7 days of coculture with irradiated NIH3T3 cells modified to stably express human CD40L, BAFF, and IL-21 (day 7; left panel ). D, The percentages of cells with a switched memory (IgD − CD27 + ) phenotype at days 0 and 7 of coculture are shown for healthy controls (HC; n = 5), I-1 (WT/R331P), P1 (R331P/R331P), and P2 (WT/G126D). E, Naive B cells were enriched as in , , and expression levels of CD38 and CD27 were assessed by flow cytometry. F, The percentages of CD27 + CD38 + cells at days 0 and 7 of coculture are shown for healthy controls (HC; n = 5), I-1 (WT/R331P), P1 (R331P/R331P), and P2 (WT/G126D). NS , Not significant. * P < .05 (2-tailed t tests).

    Journal: The Journal of allergy and clinical immunology

    Article Title: B-cell lymphocytosis and reprogramming due to biallelic CARD11 mutations

    doi: 10.1016/j.jaci.2025.12.991

    Figure Lengend Snippet: Patients with BENTA have aberrant B-cell subset distributions and show B-cell–intrinsic differentiation defects. A, IgD vs CD27 expression ( upper panel ) and CD24 vs CD38 expression ( lower panel ) were assessed within gated CD19 + B cells in PBMC samples from a healthy control (HC), I-1 (WT/R331P), P1 (R331P/R331P), and P2 (WT/G126D) by CyTOF analysis. B, Quantitation of switched memory (IgD − CD27 + ), nonswitched memory (IgD + CD27 + ), naive (CD27 − IgD + ), and transitional (CD24 hi CD38 hi ) B-cell frequencies in healthy donors, including adult healthy controls (HC, n = 12; dark blue symbols ), healthy pediatric controls (HP, n = 2; light blue symbols ), and the healthy father of P1 (WT/R331P; yellow symbol ) ( left bar ), and 2 patients with BENTA, P1 (R331P/R331P; red symbol ) and P2 (WT/G126D; orange symbol ) ( right bar ), by CyTOF analysis. * P < .05 (1-way ANOVA). C, Naive B cells were enriched from PBMC samples of 3 healthy controls (HC1, HC2, and HC3), I-1 (WT/R331P), P1 (R331P/R331P), and P2 (WT/G126D), and expression levels of IgD and CD27 were assessed by flow cytometry immediately following isolation (day 0; right panel ) and after 7 days of coculture with irradiated NIH3T3 cells modified to stably express human CD40L, BAFF, and IL-21 (day 7; left panel ). D, The percentages of cells with a switched memory (IgD − CD27 + ) phenotype at days 0 and 7 of coculture are shown for healthy controls (HC; n = 5), I-1 (WT/R331P), P1 (R331P/R331P), and P2 (WT/G126D). E, Naive B cells were enriched as in , , and expression levels of CD38 and CD27 were assessed by flow cytometry. F, The percentages of CD27 + CD38 + cells at days 0 and 7 of coculture are shown for healthy controls (HC; n = 5), I-1 (WT/R331P), P1 (R331P/R331P), and P2 (WT/G126D). NS , Not significant. * P < .05 (2-tailed t tests).

    Article Snippet: Naive B cells were enriched using the Naive B Cell Isolation Kit II, human (130–091-150; Miltenyi Biotec), MS columns (130–042-201; Miltenyi Biotec), and OctoMACS separator (130–042-109; Miltenyi Biotec) in 1× PBS + 2% FBS + 2 mM EDTA.

    Techniques: Expressing, Control, Quantitation Assay, Flow Cytometry, Isolation, Irradiation, Modification, Stable Transfection